人类Hox-4.2和Drosophila变形在Drosophila胚胎和幼虫中编码了类似的调节特异性
N McGinnis1, M A Kuziora, W McGinnis
1Department of Molecular Biophysics and Biochemistry Yale University, New Haven, Connecticut 06511.
Cell
|November 30, 1990
概括
在胚胎发育过程中,人类的Hox-4.2基因可以功能性地取代Drosophila Deformed (Dfd) 基因. 这项研究证明了跨物种的基因功能,突出了homeobox基因保留的调节作用.
科学领域:
- 发育生物学 发展生物学
- 遗传学 是一个遗传学.
- 进化生物学 进化生物学
背景情况:
- 脊椎动物的霍克斯基因复合体含有结构同异的子组与多索菲拉变形 (Dfd) 基因.
- 了解这些同源基因的功能性保护对于破译物种间的发育调节至关重要.
研究的目的:
- 调查脊椎动物Dfd类蛋白 (人类Hox-4.2) 是否可以替代Dfd在Drosophila胚胎中的调节功能.
- 为了确定人类的Hox-4.2是否可以通过转录激活Drosophila的DFD基因.
主要方法:
- 创建了一个融合基因结构,包括热冲击促进体和人类的Hox-4.2基因.
- 这种结构被引入了Drosophila基因组.
- 在胚胎和幼虫细胞中分析了热冲击诱导后的调节和发育效应.
主要成果:
- 人类Hox-4.2特别激活了Drosophila. 的内源Dfd转录单元的宫外表达.
- 人类的Hox-4.2基因复制了DFD的主导突变等位基因.
- 这些发现表明一种特定的功能替代.
结论:
- 人类Hox-4.2可以在功能上取代Drosophila Dfd基因的调节作用.
- 这证明了脊椎动物和虫之间同源的同源盒基因的保存功能.
- 强调了在发育基因调节中跨物种功能补充的潜力.
相关概念视频
The Ratio of X Chromosome to Autosomes
11.6K
In most organisms, sex is determined by the ratio of X and Y chromosomes. However, in some organisms, such as Drosophila and C.elegans, sex is determined by the ratio of the number of X chromosomes to the number of sets of autosomes. The Y chromosome in Drosophila is active but does not determine sex. It contains genes responsible for the production of sperms in adult flies.
Normal male Drosophila has a ratio of one X chromosome to two sets of autosomes. In contrast, normal female...
Normal male Drosophila has a ratio of one X chromosome to two sets of autosomes. In contrast, normal female...
11.6K
Dosage Compensation
6.4K
In animals, gender is determined by the number and type of sex chromosome. For example, human females have two X chromosomes, and males have one X and one Y chromosome, whereas C.elegans with one X chromosome is a male, and the one with two X chromosomes is a hermaphrodite.
In addition to sexual development, the X chromosome has genes involved in autosomal functions such as brain development and the immune system. Therefore, males and females with distinct numbers of X chromosomes will...
In addition to sexual development, the X chromosome has genes involved in autosomal functions such as brain development and the immune system. Therefore, males and females with distinct numbers of X chromosomes will...
6.4K
Hedgehog Signaling Pathway
7.1K
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
7.1K


