多层DNA原始体包装在一个方格格子上
Yonggang Ke1, Shawn M Douglas, Minghui Liu
1Department of Chemistry and Biochemistry, and the Biodesign Institute, Arizona State University, Tempe, Arizona 85287, USA.
Journal of the American Chemical Society
|October 8, 2009
概括
研究人员使用正方形格子开发了一个紧的3DDNA原木设计. 这一进步使得在DNA纳米技术中能够创建复杂的纳米尺度形状,提高精度和多功能性.
科学领域:
- 纳米技术 纳米技术
- 分子生物学分子生物学
- 材料科学 材料科学 材料科学
背景情况:
- DNA自组装是创建纳米结构的强大方法.
- 脚手架的DNA原形可以将长长的DNA链折叠成定制的形状.
- 之前的3D DNA原始创意使用蜂格子为折叠螺旋层.
研究的目的:
- 为了介绍一个更紧的3DDNA原木设计,基于一个方格格子.
- 为了证明3D结构的成功折叠,增加了螺旋密度.
- 提高DNA纳米技术的多功能性和范围.
主要方法:
- 用一个方格格子布局的DNA螺旋.
- 在结构折叠中采用了一步退火工艺.
- 设计定制的3D形状,具有可控制的尺寸.
主要成果:
- 通过正方形格子成功折叠了3D DNA 原木结构.
- 与以前的方法相比,证明了螺旋包装密度的增加.
- 创建精确尺寸的纳米级物体.
结论:
- 方格格子3D DNA原始设计提供了一个更紧和多功能平台.
- 这种方法有助于创建更平坦的表面和矩形结构.
- 代表了DNA纳米技术应用的基础性进步.
相关概念视频
00:58DNA Packaging
Overview
00:58DNA Packaging
Overview
00:58Genomic DNA in Eukaryotes
Eukaryotes have large genomes compared to prokaryotes. To fit their genomes into a cell, eukaryotic DNA is packaged extraordinarily tightly inside the nucleus. To achieve this, DNA is tightly wound around proteins called histones, which are packaged into nucleosomes that are joined by linker DNA and coil into chromatin fibers. Additional fibrous proteins further compact the chromatin, which is recognizable as chromosomes during certain phases of cell division.
01:32Chromatin Packaging
Each human somatic cell contains 6 billion base pairs of DNA. Each base pair is 0.34 nm long, meaning each diploid cell contains a staggering 2 meters of DNA. This long DNA strand is packed inside a nucleus measuring only 10-20 microns in diameter with the help of specialized DNA-binding proteins called histones. Together they form a compact DNA-protein complex called chromatin. The chromatin is further compacted into higher-order structures. The highest level of compaction is achieved during...
02:21Chromatin Packaging
Each human somatic cell contains 6 billion base-pairs of DNA. Each base-pair is 0.34 nm long, which means that each diploid cell contains a staggering 2 meters of DNA. How is such a long DNA strand packed inside a nucleus measuring only 10 - 20 microns in diameter?
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order structures.
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order structures.
02:05DNA as a Genetic Template
Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...