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相关概念视频

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Genome Annotation and Assembly03:36

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
Riboswitches01:56

Riboswitches

Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...

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相关实验视频

Updated: Jun 19, 2026

CorrelationCalculator and Filigree: Tools for Data-Driven Network Analysis of Metabolomics Data
07:11

CorrelationCalculator and Filigree: Tools for Data-Driven Network Analysis of Metabolomics Data

Published on: November 10, 2023

反应组阵列:在代谢组和基因组之间建立联系.

Ana Beloqui1, María-Eugenia Guazzaroni, Florencio Pazos

  • 1CSIC, Institute of Catalysis, 28049 Madrid, Spain.

Science (New York, N.Y.)
|October 10, 2009
PubMed
概括
此摘要是机器生成的。

这项研究引入了一种新的代谢物阵列,用于分析细胞和社区的代谢功能,无论基因组序列如何. 这种工具可以为已知和未测序的生物体重建代谢网络.

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High-Throughput Metabolic Profiling for Model Refinements of Microalgae
11:07

High-Throughput Metabolic Profiling for Model Refinements of Microalgae

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High-Throughput Metabolic Profiling for Model Refinements of Microalgae
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High-Throughput Metabolic Profiling for Model Refinements of Microalgae

Published on: December 4, 2021

科学领域:

  • 代谢学 代谢学 代谢学
  • 系统生物学 系统生物学
  • 生物化学 生物化学

背景情况:

  • 了解细胞代谢对于各种生物和医学应用至关重要.
  • 目前的代谢分析方法往往需要先前进行基因组测序,从而限制了它们对未被测序的生物的应用.
  • 需要一种基因组独立的方法来全面分析代谢表型和网络.

研究的目的:

  • 开发和验证一种敏感的代谢物阵列,用于细胞群和细胞群中的代谢表型和网络 (反应体) 的功能分析.
  • 为了证明数组对被测序和未测序生物的实用性.
  • 为了使全球代谢途径的重建和关键酶的识别.

主要方法:

  • 开发一个含有1676种染料连接的基质化合物的代谢物阵列,代表中心代谢途径.
  • 将细胞提取物应用于阵列,从而导致酶-基质结合,转化和信号激活.
  • 使用数组数据重建代谢地图.
  • 在纳米颗粒上捕获酶,测序和特定酶的功能建立.

主要成果:

  • 成功重建模型细菌的代谢地图.
  • 通过重建来自不同环境 (酸性火山池,深海盐水湖,碳化合物污染的海水) 的微生物群落的全球代谢,对未被测序的生物体证明了有用性.
  • 在纳米粒子上捕获的酶功能的明确确确立.

结论:

  • 代谢物阵列为代谢网络的功能分析提供了一个敏感的,独立于基因组序列的平台.
  • 这项技术显著推进了对微生物代谢学的研究,特别是对未测序的生物和复杂的群体.
  • 该阵列促进了代谢途径的重建和酶功能的表征,为系统生物学开辟了新的途径.