氧联纳米颗粒在体内检测出低酸和过氧酸生成
Peter Panizzi1, Matthias Nahrendorf, Moritz Wildgruber
1Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Simches Research Building, 185 Cambridge St., Boston, Massachusetts 02114, USA.
Journal of the American Chemical Society
|October 13, 2009
概括
研究人员开发了一种新型纳米粒子传感器,用于在体内检测活性氧/物种 (ROS/NPS). 这种成像探测器针对炎症,并提供了新的预后指标,用于组织损伤后的事件,如心脏病发作.
科学领域:
- 生物医学工程 生物医学工程
- 医疗成像医学成像
- 生物化学 生物化学
背景情况:
- 在体内对反应性氧/物种 (ROS/NPS) 进行成像是由于缺乏合适的探头而受到限制的.
- ROS/NPS与缺血引起的炎症和组织损伤有关,强调需要更好的诊断工具.
- 了解组织干预程度和ROS/NPS相关损伤的程度具有重要的临床影响.
研究的目的:
- 开发和演示一种新的化传感器,用于体内监测过氧化 (ONOO(-)) 和低酸 (HOCl/OCl(-)) 生产.
- 创建一个生物相容的纳米颗粒探针,该探针准食细胞,并在与特定的ROS/NPS反应时释放光.
主要方法:
- 制备一个纳米颗粒,涂上灭的氧化物光体.
- 在体外验证探针对各种氧化剂 (基,过氧化,超氧化物) 的稳定性.
- 使用流细胞计评估MPO依赖的探针激活和离子依赖.
- 用光反射和微观光成像在肌肉心脏梗塞后的小鼠心脏中的体内成像.
主要成果:
- 传感器可以选择性地检测过氧和MPO介导的低酸,对其他ROS保持稳定.
- 探头的激活取决于髓氧化酶 (MPO) 活性和离子,由中性粒细胞研究证实.
- 在体内成像显示,在肌肉梗塞后,小鼠心脏中含有中性粒细胞的缺血区域释放了探针.
结论:
- 开发的基于纳米粒子的化传感器使得特定ROS/NPS的目标体内成像能够实现.
- 传感器显示MPO-依赖激活,使其成为炎症相关氧化应激的特定指标.
- 这种新的ROS/NPS传感器作为一个有希望的预后指标,用于组织损伤的条件,如心肌梗塞.
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