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Updated: Jun 19, 2026

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence
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Published on: April 27, 2018

合成控制绿色光蛋白的合成控制

Kevin P Kent1, Luke M Oltrogge, Steven G Boxer

  • 1Department of Chemistry, Stanford University, Stanford, California 94305-5080, USA.

Journal of the American Chemical Society
|October 21, 2009
PubMed
概括

这项研究提出了一种创新方法,通过替换内部蛋白质段来制造半合成绿色光蛋白 (GFP). 这种方法克服了在以前的技术中看到的低产量和不完整的染色体形成.

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相关概念视频

Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...