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通过刺激辐射显微镜对具有无法检测的光的染色体进行成像

Wei Min¹, Sijia Lu, Shasha Chong

¹Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA.

|October 23, 2009

概括

刺激发射显微镜检测非光分子,克服吸收和自发发射成像的局限性. 这种新的对比机制为各种生物应用提供了高灵敏度和3D切割.

科学领域:

光学显微镜是一种光学显微镜.
生物光子学生物光子学
分子成像学分子成像学

背景情况:

许多必不可少的生物分子,如血红蛋白吸收光,但由于快速的非辐射衰变,缺乏可检测的光.
在显微镜下检测这些分子的吸收是传统方法的挑战.
自发发射虽然很敏感,但对于这些染色体来说通常是无法检测的.

研究的目的:

引入基于刺激辐射的光学显微镜的新型对比机制.
为了使以前难以成像的非光分子能够灵敏地检测.
展示刺激辐射显微镜在各种生物应用中的功能.

主要方法:

使用探针实验,其中脉冲光激发样品,时间延迟的探针脉冲刺激排放.
采用具有高兆赫频率调制的锁定放大器,用于射击噪声有限的灵敏度.
利用对输入强度的非线性依赖来实现内在3D光学切割.

主要成果:

刺激发射有效地与非辐射衰变竞争,使染色体可检测.
与自发发射或吸收相比,获得了数量级更高的灵敏度.
证明了成功的应用,包括可视化染色蛋白,监测基因表达,绘制药物分布图和无标签的血管成像.

结论:

刺激发射显微镜为光学成像提供了一个强大的新对比机制.
能够对非光生物分子和过程进行敏感,无标签的成像.
与传统的吸收和自发发射显微镜相比,它具有显著的优势,包括3D切割和高灵敏度.

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