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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Updated: Jun 19, 2026

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

隔离表面增强的拉曼散射热点使用多光子光刻法.

Eric D Diebold¹, Paul Peng, Eric Mazur

¹School of Engineering and Applied Sciences, Harvard University, 9 Oxford Street, Cambridge, Massachusetts 02138, USA.

Journal of the American Chemical Society

|October 29, 2009

概括

我们开发了一种新方法来增强使用大面积增强拉曼散射 (SERS) 基板的痕迹检测. 这种技术将分子吸附聚焦在高灵敏度的"热点"上,显著改善用于女性分子水平分析的信号强度.

科学领域:

分析化学分析化学
材料科学材料科学材料科学
频谱学是一种光谱学.

背景情况:

表面增强拉曼散射 (SERS) 能够进行敏感的分子检测.
当前的SERS基板经常遭受不均的增强,限制了灵敏度.
提高分析物分子到高增强区域的局部化是至关重要的.

研究的目的:

通过使用大面积SERS基板来增强女性分子水平的痕迹检测的新方法.
为了在物理上限制分子吸附到SERS基板上的电磁"热点".
为了改善检测到的分子的平均拉曼散射截面.

主要方法:

在SERS基板上利用商业光电阻的多光子诱导暴露.
在物理上将可用的分子吸附点限制在电磁"热点"上.
分析二醇分子在加工和未加工的SERS基板上的吸附.

主要成果:

通过改进的SERS基质,实现了女性分子水平 (10^9分子) 的痕迹检测.
光电阻过程有效地将分子吸附导向到高增强位点.
经过加工的SERS基板显示平均拉曼散射截面比未经加工的基板大27倍.

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Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

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Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons

Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons

Published on: July 21, 2018

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

结论:

开发的方法显著提高了基于SERS的痕迹检测的灵敏度.
将分子物理限制在"热点"是最大限度地提高SERS增强的有效策略.
这种技术为更强大,更敏感的分析测量提供了途径.