相关实验视频

Updated: May 5, 2026

Induction and Assessment of Class Switch Recombination in Purified Murine B Cells

Induction and Assessment of Class Switch Recombination in Purified Murine B Cells

Published on: August 14, 2010

在接受免疫球蛋白基因转换的B细胞中选择性表达RAG-2

L M Carlson¹, M A Oettinger, D G Schatz

¹Howard Hughes Medical Institute, University of Michigan Medical Center, Ann Arbor 48109.

|January 11, 1991

概括

B细胞通过基因转换使免疫球蛋白基因多样化. 研究人员发现RAG-2,而不是RAG-1,在这些细胞中被选择性地表达,这表明RAG-2对这一过程至关重要.

科学领域:

免疫学免疫学免疫学
分子生物学分子生物学
遗传学遗传学是一种遗传学.

背景情况:

免疫球蛋白 (Ig) 基因多样化通过染色体内基因转换在Fabricius的股票中发生.
新出现的证据表明,Ig基因转换和V(D) J重组之间存在联系,涉及cis作用的DNA元素.

研究的目的:

研究V(D) J重组激活基因RAG-1和RAG-2在调节Ig基因转换中的作用.
为了确定RAG-1和RAG-2是否表达在经过Ig多样化的B细胞中.

主要方法:

从Fabricius的股票中的B细胞中分析RAG-1和RAG-2mRNA表达.
突淋巴细胞和B细胞系的表征.
评估表达RAG-2mRNA的B细胞的发育和表型特征.

主要成果:

在Fabricius的股票中的B细胞表达高水平的RAG-2mRNA,但缺乏RAG-1mRNA表达.
这种选择性RAG-2表达是B细胞通过基因转换进行Ig多样化所特有的.
与哺乳动物的B细胞不同,的B细胞在这个过程中不会共同表达RAG-1和RAG-2.

结论:

在Ig基因多样化过程中,RAG-2在B细胞中通过基因转化选择性表达.
RAG-2在的Ig特定基因转换中发挥着根本性的作用.
这些发现突出了与哺乳动物相比,的Ig多样化有着独特的机制.

更多相关视频

Recombinant Retroviral Production and Infection of B Cells

Recombinant Retroviral Production and Infection of B Cells

Published on: February 19, 2011

Retroviral Overexpression of CXCR4 on Murine B-1a Cells and Adoptive Transfer for Targeted B-1a Cell Migration to the Bone Marrow and IgM Production

Retroviral Overexpression of CXCR4 on Murine B-1a Cells and Adoptive Transfer for Targeted B-1a Cell Migration to the Bone Marrow and IgM Production

Published on: May 31, 2020

相关概念视频

T Cell Activation and Clonal Selection

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...

B Cell Activation and Differentiation

B Cell Activation and Differentiation

The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...