在Caulobacter的细胞分化过程中表达位置信息
J W Gober1, R Champer, S Reuter
1Department of Developmental Biology, Stanford University School of Medicine, California 94305-5427.
Cell
|January 25, 1991
概括
在Caulobacter的细胞不对称导致不同的子细胞. 特定的鞭毛基因mRNAs被引导到蜂群细胞,由上游调节性DNA元素驱动.
科学领域:
- 细胞和分子生物学 细胞和分子生物学
- 微生物学 微生物学
- 发展生物学 发展生物学
背景情况:
- 半月菌表现出不对称的细胞分裂,产生不相似的后代细胞.
- 不同的蛋白质定位对于产生细胞多样性至关重要.
- 蜂群细胞是运动的,分化后代,而茎细胞是静止的.
研究的目的:
- 研究Caulobacter中不对称的mRNA向机制.
- 为了确定负责将mRNA分离到蜂群细胞的调节元素.
- 了解这个过程是如何对细胞命运的决定作出贡献的.
主要方法:
- 利用转录记者基因融合到鞭毛蛋白和鞭毛基因.
- 分析了Caulobacter细胞前期的mRNA局部化.
- 检查了上游调节序列 (ftr和IHF结合点) 的作用.
主要成果:
- 编码鞭毛蛋白质的记者基因mRNAs被专门分离到蜂群细胞后代中.
- mRNA分离取决于上游调节区域,而不是mRNA序列.
- 一组保留的上游cis作用元素 (ftr和IHF结合点) 与这种mRNA分离有关.
结论:
- 上游的监管要素决定了特定的鞭毛mRNA的不对称向.
- 这些元素可能有助于在预分细胞的蜂群细胞极中进行优先转录.
- 这种机制有助于Caulobacter后代的发育分歧.
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