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Updated: Jun 19, 2026

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
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Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Published on: December 11, 2021

光蛋白EosFP的光转化:混合潜力模拟研究揭示了系统间交叉

Mickaël Lelimousin1, Virgile Adam, G Ulrich Nienhaus

  • 1CNRS, UMR5075, Institut de Biologie Structurale Jean-Pierre Ebel, 41 rue Jules Horowitz, 38027 Grenoble Cedex 1, France.

Journal of the American Chemical Society
|November 6, 2009
PubMed
概括

这项研究揭示了EosFP光蛋白中绿色到红色光转换的机制. 一个兴奋状态的质子转移触发了脊柱裂变,使得超分辨率显微镜的红色光.

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相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...