Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
Combinatorial Gene Control02:33

Combinatorial Gene Control

Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Intron location and sequence modulate gene expression in Yarrowia lipolytica.

Nucleic acids research·2026
Same author

Amyloid precursor protein ortholog Appl acts with Vnd during mushroom body axon growth in Drosophila.

Genetics·2026
Same author

Non-destructive transcriptomics via vesicular export.

Nature communications·2026
Same author

Fibroblast Transcriptomics in Molecular Diagnostics of a Comprehensive Dystonia Cohort.

Annals of neurology·2026
Same author

MDGA2 homozygous loss-of-function variants cause developmental and epileptic encephalopathy.

American journal of human genetics·2026
Same author

Author Correction: The Solve-RD Solvathons as a pan-European interdisciplinary collaboration to diagnose patients with rare disease.

Nature genetics·2026

相关实验视频

Updated: Jun 19, 2026

An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations
11:36

An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

Published on: April 21, 2023

组合结合预测了时空 cis 调节活动的时空活动.

Robert P Zinzen1, Charles Girardot, Julien Gagneur

  • 1European Molecular Biology Laboratory, D-69117 Heidelberg, Germany.

Nature
|November 6, 2009
PubMed
概括
此摘要是机器生成的。

科学家们开发了一种新方法,利用转录因子结合数据来预测基因表达模式. 这种方法在开发过程中准确地解码了监管环境,而没有事先了解因子函数.

更多相关视频

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
11:34

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
12:54

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Published on: March 7, 2018

相关实验视频

Last Updated: Jun 19, 2026

An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations
11:36

An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

Published on: April 21, 2023

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
11:34

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
12:54

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Published on: March 7, 2018

科学领域:

  • 发育生物学 发展生物学
  • 基因组学就是基因组学.
  • 计算生物学 计算生物学

背景情况:

  • 精确的基因表达模式对于发育至关重要,由与cis调节模块结合的转录因子控制.
  • 全基因组转录因子占用数据可用,但解读监管格局仍然具有挑战性.

研究的目的:

  • 开发一种新的,数据驱动的方法来预测时空 cis-regulatory 活动.
  • 创建Drosophila mesoderm发育过程中的cis-regulatory模块的高分辨率地图.

主要方法:

  • 使用了体内转录因子结合和增强剂活性数据.
  • 训练有素的支向量机器使用已知的cis-regulatory模块的绑定配置文件来预测表达模式.
  • 在体内采用转基因记者测试来验证预测.

主要成果:

  • 创建了cis-regulatory模块及其时间占用情况的详细地图.
  • 成功预测了五个时空表达模式,准确度很高.
  • 在转录因子结合中观察到意想不到的可塑性,导致类似的表达结果.

结论:

  • 这种新的方法可以从转录因子结合数据准确地预测cis调节活性.
  • 这种方法具有广泛的适用性,因为它不需要对转录因子序列亲和力,功能或表达的先验知识.
  • 这些发现揭示了在发育过程中转录因子结合的可塑性.