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Nanomanipulation of Single RNA Molecules by Optical Tweezers

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Published on: August 20, 2014

想象一种催化性RNA分子的更高阶折叠

D W Celander¹, T R Cech

¹Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado, Boulder 80309-0215.

Science (New York, N.Y.)

|January 25, 1991

概括

这项研究揭示了催化RNA如何折叠成其功能形状,突出了离子在结构形成和酶活性中的关键作用. 了解 ribozyme 折叠是其催化功能的关键.

科学领域:

生物化学生物化学
分子生物学分子生物学
结构生物学结构生物学

背景情况:

催化RNA或核酶对于各种生物过程至关重要.
特拉希梅纳热的自我拼接内核是一个研究得很好的 ribozyme 模型.
了解 ribozymes 的高阶折叠对于阐明它们的催化机制至关重要.

研究的目的:

为了研究来自Tetrahymena thermophila的催化RNA的折叠过程.
确定金属离子在 ribozyme 结构和功能中的作用.
为了确定催化活性对金属离子的特定要求.

主要方法:

用Fe (II) -EDTA诱导的自由基化学物质来监测RNA折叠.
这项研究分析了不同双价金属离子 (Mg (II),Ca (II),Sr (II)) 对RNA结构的影响.
用RNA基质对催化RNA活性部位进行补充,对酶活性进行测试.

主要成果:

RNA三级结构的形成是一个需要至少三种离子的合作过程.
局部折叠过渡表现出不同的金属离子依赖性,表明特定的折叠中间体.
虽然Mg (II),Ca (II) 和Sr (II) 诱导类似的高阶折叠,但只有Mg (II) 稳定RNA具有催化活性.

结论:

二元金属离子是 ribozyme 三级结构的一般折叠的组成部分.
离子 (Mg(II)) 在这种 рибо酶的催化活性中发挥着特定和关键的作用.
这些发现阐明了 ribozyme 结构,金属离子协调和酶功能之间的关系.

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