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撤回:一种I型分泌的硫酸触发了XA21介导的先天免疫
Sang-Won Lee1, Sang-Wook Han, Malinee Sririyanum
1Department of Plant Pathology, University of California, Davis, CA 95616, USA.
概括
米植物具有XA21免疫受体,该受体识别一种称为Ax21的细菌蛋白质. 来自Ax21的特定硫酸对于激活这种植物对Xanthomonas oryzae的免疫力至关重要.
科学领域:
- 植物病理学 植物病理学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 大米的Xa21基因提供了对Xanthomonas oryzae pv.的抵抗力. 奥里扎 (Xoo) 的意思.
- 识别参与XA21介导免疫的分子成分对于理解植物防御机制至关重要.
研究的目的:
- 为了确定Xoo的特定分子,触发XA21介导的免疫力.
- 阐明XA21的分子基础,识别其细菌连接体.
主要方法:
- 液体染色学-双重质谱法 (LC-MS/MS) 用于分析Xoo的超.
- 进行了共免疫沉试验,以研究蛋白质相互作用.
- 从已识别的蛋白质中提取的合成被测试了生物活性.
主要成果:
- 一种由194个氨基酸组成的蛋白质,被指定为Ax21,被确定为Xoo超水生物中的活性成分.
- 来自Ax21的N端的17-氨基酸硫酸 (axY(S) 22足以引起XA21介导的免疫力.
- 该的硫化对其生物活性至关重要.
- XA21直接与 axY(S) 22 结合,正如共免疫沉所示.
- 在Xanthomonas物种中保留了axY(S) 22.
结论:
- Ax21作为一种病原体相关分子模式 (PAMP) 起作用.
- XA21作为一种模式识别受体 (PRR) 起作用,该受体特别识别硫化 axY(S) 22 .
- 这一发现澄清了基于XA21的植物免疫力与细菌病原体之间的分子相互作用.
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