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相关概念视频

Organization of Genes02:07

Organization of Genes

Overview
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Exon Recombination02:32

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

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相关实验视频

Updated: Jun 25, 2026

Structure-function Studies in Mouse Embryonic Stem Cells Using Recombinase-mediated Cassette Exchange
15:13

Structure-function Studies in Mouse Embryonic Stem Cells Using Recombinase-mediated Cassette Exchange

Published on: April 27, 2017

被编码的前子被编码了.

J M Nigro1, K R Cho, E R Fearon

  • 1Oncology Center, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231.

Cell
|February 8, 1991
PubMed
概括
此摘要是机器生成的。

研究人员发现了杂乱的RNA转录,在这种转录中,基因外基因在拼接过程中失序. 这种新型的RNA产品形成挑战了对基因表达和RNA处理的传统理解.

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Using the E1A Minigene Tool to Study mRNA Splicing Changes
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Electroporation-Based CRISPR-Cas9-Mediated Gene Knockout in THP-1 Cells and Single-Cell Clone Isolation
09:29

Electroporation-Based CRISPR-Cas9-Mediated Gene Knockout in THP-1 Cells and Single-Cell Clone Isolation

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相关实验视频

Last Updated: Jun 25, 2026

Structure-function Studies in Mouse Embryonic Stem Cells Using Recombinase-mediated Cassette Exchange
15:13

Structure-function Studies in Mouse Embryonic Stem Cells Using Recombinase-mediated Cassette Exchange

Published on: April 27, 2017

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Electroporation-Based CRISPR-Cas9-Mediated Gene Knockout in THP-1 Cells and Single-Cell Clone Isolation
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Electroporation-Based CRISPR-Cas9-Mediated Gene Knockout in THP-1 Cells and Single-Cell Clone Isolation

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科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 在RNA分离过程中.

背景情况:

  • 替代RNA拼接是产生蛋白质多样性的关键机制.
  • 在拼接过程中,外显子连接的精确顺序对于产生功能转录至关重要.
  • 候选瘤抑制基因DCC与细胞生长和发育有关.

研究的目的:

  • 调查异常RNA拼接事件的发生和性质.
  • 为了识别由非顺序的外因子结合产生的新型RNA产物.
  • 了解正常和瘤细胞中编码转录的含义.

主要方法:

  • 敏感的RNA表达试验被用来检测不寻常的转录.
  • 用聚合酶链反应 (PCR) 放大来分离特定的RNA段.
  • 使用克隆和测序技术来确定异常转录的确切结构.

主要成果:

  • 鉴定出了多个DCC基因的异常拼接的转录.
  • 这些转录中的前基因在拼接地点准确地连接在一起,但是在一个混乱的顺序.
  • 描述了四种不同类型的编码转录,每种都涉及不同的表子对.
  • 在各种正常和癌细胞中,低水平的编码转录被检测到,主要是在非多聚化细胞质RNA中.

结论:

  • 拼接机器可以以非顺序的顺序连接外子,偏离基因组DNA排列.
  • 这一过程产生了具有潜在改变功能的新型RNA产品.
  • 这些发现揭示了RNA处理的以前未知的机制,对基因表达调节有影响.