一个转化作用的核糖转换器控制了Listeria monocytogenes中毒性调节器PrfA的表达
Edmund Loh1, Olivier Dussurget, Jonas Gripenland
1Department of Molecular Biology, Umeå University, 90187 Umeå, Sweden.
Cell
|November 17, 2009
概括
在Listeria monocytogenes中,两个S-adenosylmethionine (SAM) рибо开关SreA和SreB可以在转基因中发挥作用,通过控制PrfA调节器来调节细菌毒性基因表达. 这揭示了一个新的非编码RNA类别,将营养素的可用性与毒性联系起来.
科学领域:
- 细菌学 细菌学是一门学科.
- 分子生物学分子生物学
- 在RNA生物学,RNA生物学.
背景情况:
- рибо开关是cis作用的RNA元素,通过代谢物诱导的结构变化调节基因表达.
- S-adenosylmethionine (SAM) 是一个关键的代谢物,参与各种细胞过程.
- 细菌的毒性通常由复杂的遗传网络来调节.
研究的目的:
- 调查SAM рибо开关SreA和SreB在Listeria monocytogenes中的调控作用.
- 为了确定这些 рибо开关是否可以作为非编码RNA在转基因中发挥作用.
- 为了阐明营养可用性和细菌毒性之间的联系.
主要方法:
- 在Listeria monocytogenes中SreA和SreB的遗传分析.
- 对病毒毒性调节剂PrfA.的mRNA和蛋白质表达分析.
- 报告员测试以评估 рибо交换机活动.
主要成果:
- 发现SreA和SreB在转基因中起作用,作为调控性非编码RNA.
- 这些 рибо开关与PrfA mRNA的5'-untranslated区域结合.
- 删除SreA和SreB导致PrfA水平增加,并增强病毒性基因表达.
结论:
- 在Listeria monocytogenes中,SreA和SreB的SAM рибо开关起着一种新的跨作用调节作用.
- 这些 рибо开关将细胞内SAM水平与细菌毒性的调节联系起来.
- 一个新的调节性非编码RNA类被发现,它们与细菌病原发生有关.
相关概念视频
01:23Types of RNA
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
01:58Prokaryotic Transcriptional Activators and Repressors
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Transcription of prokaryotic...
01:56Riboswitches
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
01:58Prokaryotic Transcriptional Activators and Repressors
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Transcription of prokaryotic...
01:23Transcriptional Regulation: Riboswitches
Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
01:29Translational Regulation
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...