蛋白质质子转移在短键潜在能量表面的超快动态:S65T/H148D GFP
Minako Kondo1, Ismael A Heisler, Deborah Stoner-Ma
1School of Chemistry, University of East Anglia, Norwich NR4 7TJ, UK.
Journal of the American Chemical Society
|November 18, 2009
概括
绿色光蛋白 (GFP) 突变体中的超快速质子转移发生在100 femtosecond内. 这种快速的质子转移之后会出现更慢的振动冷却和在皮秒时间尺度上的结构变化.
科学领域:
- 生物物理学的生物物理.
- 摄影化学的使用.
- 蛋白质动力学 蛋白质动力学
背景情况:
- 绿色光蛋白 (GFP) 是分子生物学中的一个重要工具.
- 质子转移对于许多生物过程至关重要.
- 了解蛋白质的超快动态是阐明它们功能的关键.
研究的目的:
- 为了研究特定的GFP突变体中的超快质子转移动态.
- 为了将质子转移与染色体和宿主结构变化相关联.
- 为了确定这些动态事件的时间尺度.
主要方法:
- 使用时间分辨率光光谱学.
- 使用了一种S65T/H148D绿色光蛋白 (GFP) 突变体.
- 探测了五秒和皮秒的时间尺度.
主要成果:
- 质子转移发生在小于100 femt秒的时间尺度上.
- 染色体pK (a) 在激发时发生的变化启动了转移.
- 图秒时间尺度显示了振动冷却和宿主结构重组.
结论:
- 这项研究阐明了GFP中的快速质子转移机制.
- 五秒动态与蛋白质功能直接相关.
- 这项研究提供了对蛋白质能量消散途径的见解.
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