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相关概念视频

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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相关实验视频

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Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays
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Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Published on: November 13, 2017

基于小分子的结合环境:微阵列的组合构造用于多重亲和力选.

Rachel L Weller Roska1, Tenzing Gawa Surshar Lama, Jay P Hennes

  • 1RECEPTORS LLC, 1107 Hazeltine Boulevard, Suite 510, Chaska, Minnesota 55318, USA.

Journal of the American Chemical Society
|November 19, 2009
PubMed
概括

开发了一种新的组合人工受体阵列 (CARA),用于蛋白质分化. 这种微阵列技术能够使用各种合成受体快速分析蛋白质结合模式.

科学领域:

  • 化学生物学 化学生物学
  • 材料科学 材料科学 材料科学
  • 生物技术是生物技术.

背景情况:

  • 在化学生物学中,开发用于分子识别的多功能平台至关重要.
  • 蛋白质分化依赖于特定的结合相互作用,需要先进的测试技术.

研究的目的:

  • 构建和验证用于蛋白质分化的组合人工受体阵列 (CARA).
  • 为了证明CARA在区分基于其独特结合模式的蛋白质的能力.

主要方法:

  • 使用19个小分子构建块制造具有5035个独特结合环境的微阵列.
  • 构建块的共价固定在氨基功能化玻璃幻灯片上,通过碳酸柄.
  • 使用CARA化标记蛋白质 (ubiquitin,myoglobin,α-1-酸糖蛋白,lyszyme) 的化.

主要成果:

  • 成功构建一个灵活和适应性的CARA平台.
  • 在整个数组中证明了蛋白质结合模式的可复制性.
  • 根据它们的CARA结合配置文件,在四种不同的蛋白质之间实现了显著的差异化.

结论:

  • 卡拉战略为受体合成和阵列构建提供了可扩展和灵活的方法.

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Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library
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Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions
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Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

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Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library
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Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions
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Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Published on: January 7, 2019

  • 卡拉技术为蛋白质分析和分化提供了强大的工具.
  • 开发的绑定环境是稳定的,可以适应各种格式.