在多基基生物合成中的立体化学控制的生物化学基础
Chiara R Valenzano1, Rachel J Lawson, Alice Y Chen
1Department of Chemistry, Box H, Brown University, Providence, Rhode Island 02912-9108, USA.
聚基化合成酶通过减酶域控制立体化学. 在链延长后发生甲基组表皮化,由缩酶域本身催化.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 有机化学 有机化学
背景情况:
- 复杂的多基基体具有众多含甲基和基的立体中心.
- 了解多基基体立体化学的生物化学控制是至关重要的.
研究的目的:
- 调查多基类立体化学控制的生物化学基础.
- 确定甲基载体中心的表皮化时间和机制.
主要方法:
- 使用了来自模块化多基酸合成酶的复合组分.
- 进行了一系列的化,以分析产品形成和立体化学.
主要成果:
- 产品的立体化学与脱酶域内在的立体特异性直接相关.
- 在合成酶催化链延长后,甲基组的表皮化发生.
- 经皮化很可能是由缩酶域催化,但观察到的速率差异很小.
结论:
- 缩酶域是多基基体立体化学的关键决定因素.
- 甲基载体中心的表皮化是一种延长后的过程.
- 减酶域本身催化了表皮化反应.
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