在Drosophila兄弟物种中,通过混合无菌蛋白改变了异性染色素的结合
Joshua J Bayes1, Harmit S Malik
1Molecular and Cellular Biology Program, University of Washington, Seattle, WA 98185, USA.
Science (New York, N.Y.)
|November 26, 2009
概括
杂交的男性不育性源于奥德赛乌斯网站本地盒 (OdsH) 基因. 异性染色素的快速演变影响了OdsH.
科学领域:
- 遗传学和进化生物学
- 生殖隔离机制 生殖隔离机制
- 规格研究研究 规格研究
背景情况:
- 异性恋性别中的杂交不育是一种早期演变的后异位生殖障碍.
- 混合不孕症的分子基础在很大程度上仍然未被阐明.
- 了解这些障碍对于理解物种化至关重要.
研究的目的:
- 为了研究杂交男性不孕不育的分子基础.
- 为了确定奥德赛乌斯网站本地盒 (OdsH) 基因在这个过程中的作用.
- 探索异性染色体进化的对生殖隔离的影响.
主要方法:
- 基因识别和特征的奥德赛乌斯网站本地盒 (OdsH).
- 在异色彩区域内的蛋白质局部化研究.
- 在Drosophila杂交雄性 (D. mauritiana x D. simulans) 中对OdsH功能的比较分析.
- 在无菌杂交丸中检查精子生成.
主要成果:
- OdsH基因编码了一种蛋白质,该蛋白质局部化到动态的异色谱位点,导致脱凝.
- 在D. mauritiana x D. simulans雄性混合体中,D. mauritiana OdsH (OdsHmau) 通过与D. simulans Y染色体相互作用,起到杀菌因子的作用.
- D. simulans OdsH (OdsHsim) 不会导致不孕.
- 在精子生成过程中OdsH丰度和局部化的差异与杂交不育相关.
结论:
- 异性染色素的快速进化是混合男性不孕症发生的关键因素.
- 奥德赛乌斯本地本地盒 (OdsH) 基因在通过异色素蛋白相互作用调解杂交不孕症方面发挥着关键作用.
- 物种之间的差异性OdsH功能有助于在物种化期间的繁殖隔离.
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