宿主植物基因组克服了对共生固定的细菌基因缺乏
Tsuneo Hakoyama1, Kaori Niimi, Hirokazu Watanabe
1National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan.
Nature
|November 27, 2009
概括
日本莲花FEN1基因编码 homocitrate synthase (HCS),使没有NifV.的豆类能够固定. 宿主衍生的同酸盐对于共生树枝生物中的酶合成至关重要.
科学领域:
- 植物 - 微生物共生.
- 固化的生物化学
背景情况:
- 固定对于植物生长至关重要.
- 类同位素合成酶 (HCS) 对于酶活性至关重要.
- 大多数根茎菌缺乏HCS的NifV基因,但可以进行共生固化.
研究的目的:
- 研究莲花japonicus FEN1基因在共生固定中的作用.
- 确定FEN1是否可以弥补根茎菌中NifV缺乏的情况.
主要方法:
- 具有无效结节的植物突变 (fen1) 的特征.
- 补充了一个HCS缺陷酵母突变体与FEN1基因.
- 在用修改后的根茎菌或提供外源性同酸盐的fen1突变体接种后评估固活性.
主要成果:
- 1突变体形成了缺乏同类酸盐的无效结节.
- FEN1编码HCS,并补充缺乏HCS的酵母.
- 在fen1结节中恢复了FEN1或NifV的固定.
- 外源性同位素在结体中挽救了固定.
结论:
- 宿主衍生的同位素是基酶合成在根菌的bacteroids必不可少.
- 豆类FEN1基因提供HCS活性,使共生固定成为可能.
- 这突出了补充性伙伴关系,这对于豆类-树枝生物共生至关重要.
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