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Updated: Jun 18, 2026

Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability
10:31

Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability

Published on: February 3, 2022

单个氨基酸的替换使得Aequorea victoria的光蛋白可逆地变光

Ranieri Bizzarri1, Michela Serresi, Francesco Cardarelli

  • 1IIT@NEST, Center for Nanotechnology Innovation, Piazza San Silvestro 12, I-56127 Pisa, Italy. r.bizzarri@sns.it

Journal of the American Chemical Society
|December 5, 2009
PubMed
概括

研究人员开发了四种新的光色光蛋白 (AFP) 用于先进的生物成像. 一个单一的突变使明亮和黑暗状态之间的可逆切换成为可能,增强了像FRET这样的活细胞成像技术.

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Structure Solution of the Fluorescent Protein Cerulean Using MeshAndCollect06:42

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Channel Rhodopsins01:11

Channel Rhodopsins

Most organisms use photoreceptors to sense and respond to light. Examples of photoreceptors include bacteriorhodopsins and bacteriophytochromes in some bacteria, phytochromes in plants, and rhodopsins in the photoreceptor cells of the vertebral retina. The light-sensitive property of these receptors is because of the bound chromophores, such as bilin in the phytochromes and retinal in the rhodopsins.
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...
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Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...