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Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors
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使用光子晶体生物传感器识别蛋白质-蛋白质相互作用的调节器

James T Heeres1, Seok-Ho Kim, Benjamin J Leslie

  • 1Department of Biochemistry, University of Illinois, Urbana, Illinois 61801, USA.

Journal of the American Chemical Society
|December 9, 2009
PubMed
概括

这项研究引入了一种高通量光子晶体生物传感器试验,用于识别蛋白质-蛋白质相互作用调节器. 该试验使用一种用于蛋白质固定的新型化合物,并检测结合事件,从而实现高效的药物发现.

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Protein-protein Interfaces02:04

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
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Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...