家长印记的小鼠胰岛素样生长因子II基因
T M DeChiara1, E J Robertson, A Efstratiadis
1Department of Genetics and Development, Columbia University, New York, New York 10032.
Cell
|February 22, 1991
概括
父母对胰岛素样生长因子II (IGF-II) 基因的印记会影响胚胎的生长. 突变IGF-II等位基因的父亲传播会导致小鼠的生长缺陷,而母亲传播不会.
科学领域:
- 遗传学 是一个遗传学.
- 发展生物学 发展生物学
- 基因组印记是一种基因组印记.
背景情况:
- 胰岛素样生长因子II (IGF-II) 在胚胎发育中起着至关重要的作用.
- 父母印记,只表达一个父母等位基因,是关键的表观遗传机制.
- 了解IGF-II的印记对于理解增长调节至关重要.
研究的目的:
- 研究IGF-II在胚胎生长中的作用.
- 为了确定IGF-II突变对后代表型的父母起源的影响.
- 为了阐明IGF-II等位基因的组织特异性表达模式.
主要方法:
- 产生具有针对性破坏IGF-II基因的小鼠.
- 基于父母传播的异卵性后代的表型差异分析.
- 核酶保护和现场杂交以评估等位基因特异性的基因表达.
主要成果:
- IGF-II突变的从母亲传播导致了表型正常的异构卵性后代.
- 父亲传播IGF-II突变导致生长缺陷的异质合体后代.
- 父亲和母亲的IGF-II等位基因都表达在冠状动脉和膜中,但只有父亲的等位基因在其他胚胎组织中表达.
结论:
- IGF-II对于正常的胚胎生长至关重要.
- IGF-II基因表现出特定于组织的父母印记.
- 父母印记的IGF-II显著影响胚胎发育和生长表型.
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