一个Arabidopsis Polycomb目标的长时间反意义转录的冷诱导沉默
Szymon Swiezewski1, Fuquan Liu, Andreas Magusin
1John Innes Centre, Colney, Norwich, NR4 7UH, UK.
Nature
|December 17, 2009
概括
在Arabidopsis thaliana的FLC基因中,冷诱导的非编码反意义转录可能会启动表观遗传沉默. 这些转录似乎暂时减少了FLC感官转录,在Polycomb机械招募长期记忆之前.
科学领域:
- 植物分子生物学 植物分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 转录条例 转录条例 转录条例
背景情况:
- 细胞转录产生了大量的非蛋白质编码RNA,其功能在很大程度上是未知的.
- 阿拉比多普西斯·塔利亚纳 (Arabidopsis thaliana) 中的FLC基因调节了开花时间,是染色质通路的目标.
- 了解非编码RNA在染色质调节中的作用至关重要.
研究的目的:
- 使用FLC基因研究非编码RNA和染色质调节之间的联系.
- 描述FLC的非编码转录及其在冷诱导表观遗传沉默中的作用.
主要方法:
- 没有偏见的策略来表征FLC非编码转录.
- 在各种突变物和治疗中分析感觉/反感觉转录水平,包括寒冷.
- 记者基因测试来测试FLC反意义促进子序列的功能.
主要成果:
- 感觉和反感觉的FLC转录水平通常是相关的,但在冷处理植物中独立变化.
- 覆盖FLC位点的长非编码反意义转录的升级与冷暴露相吻合,并且先于其他本地化标记.
- FLC反感应转录引发了FLC感应转录的暂时沉默,它们的促进子序列给记者基因带来了冷诱导的沉默.
结论:
- 冷诱导的FLC反感应转录在FLC的表观遗传沉默中起着早期的作用,作用是暂时减少感觉转录.
- 这种反感应转录可能是冷感应机制的早期部分,Polycomb机器赋予了表观遗传记忆.
- 从3'基因末端的反意义转录可能是条件依赖感觉转录调节的一般机制.
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