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Updated: Jun 17, 2026

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
13:48

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Published on: May 29, 2012

用拉曼显微镜测定活细胞中的透二次结构

Jing Ye1, Sara A Fox, Mare Cudic

  • 1Department of Chemistry and Biochemistry, Florida Atlantic University, Boca Raton, Florida 33431, USA.

Journal of the American Chemical Society
|January 1, 2010
PubMed
概括

我们开发了一种新的拉曼显微镜方法,用于追踪活细胞中的细胞透 (CPP). 这种技术揭示了CPPs.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...