相关实验视频
Updated: Apr 29, 2026
07:40
Dissection and Staining of Drosophila Larval Ovaries
Published on: May 13, 2011
25.9K
一个短暂的利基调节了Drosophila肠道干细胞的规范
Divya Mathur1, Alyssa Bost, Ian Driver
1Department of Genetics and Development, Columbia University Medical Center, New York, NY 10032, USA.
Science (New York, N.Y.)
|January 9, 2010
概括
干细胞利基对于干细胞命运至关重要. 在Drosophila中,原始细胞使用Notch信号和去角性指导性肠干细胞 (ISC) 规范创建暂时的.
科学领域:
- 发展生物学 发展生物学
- 干细胞生物学 干细胞生物学
- 德洛索菲拉 (Drosophila melanogaster) 的研究研究
背景情况:
- 干细胞是干细胞维护和自我更新的必要微环境.
- 控制干细胞最初建立的机制,特别是在成年组织中,仍然在很大程度上未被探索.
- 成人的Drosophila中肠依赖肠道干细胞 (ISC) 进行组织稳定.
研究的目的:
- 调查在成年Drosophila中肠中肠中肠干细胞 (ISC) 建立的未知过程.
- 在ISC规范中阐明原生细胞和信号通路的作用.
- 在干细胞发育过程中探索干细胞与利基相互作用的新范式.
主要方法:
- 使用了成年Drosophila中肠模型系统.
- 研究了Notch信号在ISC祖先行为中的作用.
- 检查了骨形态遗传蛋白质同类体的dkapentaplegic (Dpp) 在利基形成和祖先维护中的功能.
主要成果:
- 证明肠干细胞 (ISC) 的祖先通过Notch信号产生一个利基细胞.
- 表明这种短暂的利基利用脑 (Dpp) 维持祖先不分化.
- 揭示了利基分解和亡导致成年中肠中的ISCs的规范.
结论:
- 提出了干细胞利基生物学的新模型,其中由祖先生成过渡性利基以建立干细胞.
- 突出了Notch信号和DPP在编排ISC规范中的关键作用.
- 表明这种短暂的利基介导干细胞建立机制可能在其他组织中得到保存.
相关概念视频
01:33Adult Stem Cells
27.8K
Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
27.8K
01:26Stem Cell Niche
5.0K
The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
5.0K
01:23Renewal of Intestinal Stem Cells
2.7K
The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...
2.7K
01:22Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal
1.8K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
1.8K
01:06Multipotency and Niche of Bulge Stem Cell
2.9K
A hair follicle or HF is a small part of the skin that produces the hair shaft. Paul Gerson Unna was the first to observe a bulge in the human hair follicle's outer root sheath (ORS). The bulge is present between the sebaceous gland and the arrector pili muscle and is the niche for hair follicle stem cells (HFSCs). The bulge is also a niche for melanocyte stem cells, and their loss results in graying of hair. The HFSCs express Sox9 and Lhx2, which help them maintain stemness and prevent...
2.9K
01:12Role Of Notch Signalling In Intestinal Stem Cell Renewal
1.8K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
1.8K