铜离子在调节结合因子结合中的作用,在基于髓红蛋白的细胞染色体C氧化酶模型中
Changyuan Lu1, Xuan Zhao, Yi Lu
1Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
Journal of the American Chemical Society
|January 15, 2010
概括
在这项研究中,设计了一个肌球蛋白突变体 (Cu(B) Mb,作为细胞染色体c氧化酶 (CcO) 的最小模型. Cu(B) Mb模型模仿了CcO的结构和连接体结合动力学,特别类似于Thermus thermophilus CcO.
科学领域:
- 生物化学 生物化学
- 生物有机化学 生物有机化学
- 酶学 是一种酶学.
背景情况:
- 细胞染色体c氧化酶 (CcO) 对于细胞呼吸至关重要,减少氧气到水.
- CcO具有双核中心,其中含有海姆a(3) 和铜原子 (Cu(B)).
- 了解CcO的机制需要简化模型.
研究的目的:
- 在最小CcO模型中研究铜在调节连接体结合中的作用.
- 为了将模型的连接体结合动力学与不同的CcO酶进行比较.
主要方法:
- 设计了一种精液的肌球蛋白突变体 (Cu(B) Mb,在远端血口袋中有一个铜原子.
- 利用共振拉曼光谱来研究血质环境.
- 采用停止流动动力学来分析连接物 (CO,NO) 的结合和解离.
主要成果:
- Cu(B) Mb 呈现出类似于 CcO 的远端血口袋结构.
- 带结合始于Cu(+) 并且通过分子内转移到Fe(2+),模仿CcO.
- Cu(B) Mb/Cu(+) 的动力性质与Thermus thermophilus CcO (TtCcO) 相一致,而不是牛的CcO (bCcO).
- 光解研究表明,短暂的铜结合和完全的双重组合,类似于TtCcO.
结论:
- 复合 Cu(B) Mb/Cu(+) 作为一个有效的最小模型,用于 CcO.
- 该模型复制了CcO双核中心的关键结构和运动特征.
- Cu(B) Mb/Cu(+) 与TtCcO相比,与bCcO的相似性更大,可以了解酶变异.
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