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Updated: Jun 17, 2026

Spatiotemporally Controlled Nuclear Translocation of Guests in Living Cells Using Caged Molecular Glues as Photoactivatable Tags
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光介导的空间控制通过光性光灭的酸盒

Hsien-Ming Lee1, Melanie A Priestman, David S Lawrence

  • 1Department of Chemistry, Division of Medicinal Chemistry and Natural Products, University of North Carolina, Chapel Hill, North Carolina 27599, USA.

Journal of the American Chemical Society
|January 16, 2010
PubMed
概括

研究人员开发了新的光敏感分子探针,在光激活时显著增强光. 这些探针能够精确控制线粒体内的生物活动,为细胞研究提供了一个新的工具.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...