植物染色体到激活的Pfr形式的光转化结构基础
Andrew T Ulijasz1, Gabriel Cornilescu, Claudia C Cornilescu
1Department of Genetics and, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
Nature
|January 16, 2010
概括
植物染色体,感光蛋白,在状态之间切换,以控制植物的反应. 这项研究揭示了Pfr结构,表明光诱导的染色体旋转启动了信号通路.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 摄影生物学 摄影生物学
背景情况:
- 植物染色体是含有比林的光受体,调节植物和微生物的光反应.
- 它们在红光 (Pr) 和远红光 (Pfr) 状态之间切换,但Pfr的结构和信号传输不太清楚.
研究的目的:
- 确定在光激活的Pfr状态下,蓝藻细菌植物染色体的比林结合域的三维溶液结构.
- 阐明植物染色信号发射的结构基础.
主要方法:
- 核磁共振 (NMR) 光谱法用于确定溶液结构.
- 分析的重点是Synechococcus OSB'植物染色体的比林结合域.
主要成果:
- Pfr状态结构揭示了A环的光诱导的旋转,而不是D环,作为主要的染色体运动.
- 蛋白质的重新排列会影响植物染色体二次体内的域内和域间接触.
结论:
- 植物染色体通过传输光驱动的 bilin染色体的形状变化来发出信号.
- 由这些变化驱动的输出域之间的接触改变,可能会直接下游信号事件,如光转移.
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