含有H2A.Z的核体调解了Arabidopsis中的热感应反应
S Vinod Kumar1, Philip A Wigge
1John Innes Centre, Colney Lane, Norwich NR4 7UH, UK.
Cell
|January 19, 2010
概括
核细胞中的替代组素H2A.Z对于植物感知环境温度至关重要. 这种机制涉及DNA - 核酶体波动,在酵母中保存,并根据温度调节基因表达.
科学领域:
- 植物分子生物学 植物分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 热感应机制的热感应机制
背景情况:
- 植物对环境温度具有很高的敏感性,能够感知到1°C的变化.
- 温度感知和其融入植物发展的基础分子机制在很大程度上是未知的.
研究的目的:
- 为了确定参与Arabidopsis温度感知的遗传因素.
- 阐明特定的组织蛋白变体在热传感中的作用.
主要方法:
- 在Arabidopsis中转发基因选,以识别具有改变温度反应的突变物.
- 核细胞组合的分析,特别是基 H2A.Z. 的结合.
- 转录组分析以评估不同温度条件下的基因表达变化.
- 使用净化核体的生物物理研究来研究DNA-核体相互作用.
主要成果:
- 缺少H2A.Z核细胞组合的突变者表现出一个构成性的温暖温度转录组.
- 含有H2A.Z的核细胞体在体外表现出温度依赖的DNA解封特性,独立于转录.
- H2A.Z核细胞提供直接的热传感信息,协调环境温度的转录.
结论:
- 含有替代基因素H2A.Z的核子对植物中准确的环境温度感知至关重要.
- 通过H2A.Z介导的DNA解封提供了一个直接的机制,用于核细胞层面的温度传感.
- 这种热感应机制在进化过程中得到了保护,在芽酵母中观察到的类似效应证明了这一点.
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