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相关概念视频

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Replicative Cell Senescence02:15

Replicative Cell Senescence

Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds the telomeric...
Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
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Satellite Stem Cells and Muscular Dystrophy

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相关实验视频

Updated: Jun 17, 2026

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
09:42

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

在自我更新的体组织中,DNMT1维持了原始细胞的功能.

George L Sen1, Jason A Reuter, Daniel E Webster

  • 1Programs in Epithelial Biology and Cancer Biology and the Stanford Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, Stanford, California 94305, USA.

Nature
|January 19, 2010
PubMed
概括
此摘要是机器生成的。

基因甲基转移酶1 (DNMT1) 对于维持表皮原生细胞,防止过早分化和组织损失至关重要. 缺少它会破坏DNA甲基化平衡,影响细胞更新和组织健康.

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Utilizing Murine Inducible Telomerase Alleles in the Studies of Tissue Degeneration/Regeneration and Cancer
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Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells
11:06

Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells

Published on: February 24, 2014

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Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
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Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

Utilizing Murine Inducible Telomerase Alleles in the Studies of Tissue Degeneration/Regeneration and Cancer
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Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells
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科学领域:

  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
  • 细胞生物学 细胞生物学
  • 皮肤病学 皮肤病学

背景情况:

  • 主细胞对于组织更新至关重要,需要增殖和抑制分化.
  • 基因甲基化是一种表观遗传机制,可能与维持原始细胞身份有关.
  • 对于DNA甲基转移酶1 (DNMT1) 在体质原始细胞维持中的作用尚不清楚.

研究的目的:

  • 研究DNMT1在维持表皮原生细胞功能中的重要作用.
  • 阐明DNMT1调节祖细胞增殖和分化的机制.
  • 了解体质组织更新中的DNA甲基化动态调节.

主要方法:

  • 在未分化的表皮细胞中对DNMT1蛋白质丰富的分析.
  • 在哺乳动物表皮中减少DNMT1,以观察对原生细胞的影响.
  • 对差异化基因促进者的DNA甲基化模式的全基因组分析.
  • 研究UHRF1,Gadd45A和Gadd45B在表皮原生细胞调节中的作用.

主要成果:

  • DNMT1对于表皮原生细胞功能,增殖和抑制分化至关重要.
  • DNMT1的枯竭导致原生细胞退出隔间,导致过早的分化和组织损失.
  • 皮肤分化基因促进体在自我更新的细胞中甲基化,并在分化过程中脱甲基化.
  • UHRF1是抑制过早分化所必需的,而Gadd45A/B则促进分化基因诱导.

结论:

  • DNMT1对于维持哺乳动物表皮的原始状态至关重要.
  • 通过DNMT1和UHRF1等蛋白质对DNA甲基化的动态调节对于原始细胞的维持和自我更新至关重要.
  • 通过Gadd45A/B调解的活性DNA去甲基化是适当的表皮分化所需的.