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人类HOXD集群的一个区域,它赋予了多组响应能力
Caroline J Woo1, Peter V Kharchenko, Laurence Daheron
1Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA.
Cell
|January 21, 2010
概括
研究人员在人类胚胎干细胞中发现了一种新型的Polycomb响应元素 (PRE). 这个D11.12区域的目标是聚合组 (PcG) 蛋白质,对于胚胎发育和基因调节至关重要.
科学领域:
- 发展生物学 发展生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 基因组学就是基因组学.
背景情况:
- 多组 (PcG) 蛋白质对于胚胎轴向模式至关重要.
- 聚合体反应元素 (PREs) 准Drosophila中的PcG介导抑制,但在人类中仍然未被描述.
研究的目的:
- 在胚胎干细胞分化过程中识别和表征向PcG蛋白的人类序列.
- 研究和哺乳动物之间PcG向机制的功能性保护.
主要方法:
- 在分化过程中对人类胚胎干细胞 (hESCs) 的染色质结构进行分析.
- 在HOXD11和HOXD12.12之间识别1.8kb区域 (D11.12).
- 报告者测试使用光酶表达来评估调节功能.
- 评估蛋白质依赖性,包括PcG蛋白 (BMI1,EED) 和YY1相互作用伙伴 (RYBP).
主要成果:
- 在HOXD11和HOXD12之间的1.8kb区域 (D11.12) 被确定并与PcG蛋白相关.
- 在hESC分化过程中,D11.12元素表现出核酶敏感性的动态变化.
- D11.12抑制的露西法酶表达,完全抑制取决于一个保存区域,YY1结合点,BMI1,EED和RYBP.
- 这些发现表明,D11.12功能作为一个聚合体依赖的调节区域.
结论:
- D11.12 是人类胚胎干细胞中一个新型的多依赖性调节区域.
- 这个元素与Drosophila PREs具有功能上的相似性,表明保留了PcG向机制.
- 这一发现为哺乳动物胚胎发育的表观遗传调节提供了洞察力.
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