在干燥耐受性中ABA和ABI3的作用
A Khandelwal1, S H Cho, H Marella
1Monsanto Company, 700 Chesterfield Parkway West, St. Louis, MO 63017, USA.
概括
酸 (ABA) 预处理使的细胞具有干燥耐受性,类似于血管种. 转录调节器ABSCISIC ACID INSENSITIVE 3 (ABI3) 对于这种ABA介导的保护和恢复至关重要.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 压力生理学 压力生理学
背景情况:
- 耐干燥对植物在干旱环境中的生存至关重要.
- 在开花植物 (芽植物) 中,酸 (ABA) 和酸无敏3 (ABI3) 是种子干燥耐受性的关键调节剂.
- ABA和ABI3在非种子植物 (如植物) 的干燥耐受性中的作用不太清楚.
研究的目的:
- 调查酸 (ABA) 和无敏酸3 (ABI3) 在赋予植物 (Physcomitrella patens) 干燥耐受性的作用.
- 为了识别在中干燥和恢复期间由ABA和ABI3调节的基因.
主要方法:
- 用ABA预处理了 (*Physcomitrella patens*) 细胞.
- 在野生类型和*ABI3*删除线 (DeltaPpabi3) 中评估了ABA治疗疗效.
- 用RNA测序进行基因表达分析,以识别ABA诱导的基因和ABI3标.
主要成果:
- 通过ABA预处理,野生类型的细胞成功地获得了干燥耐受性.
- ABA未能保护*ABI3*删除线,表明ABI3对于中ABA介导的干燥耐受性至关重要.
- 22个基因被确定为ABA诱导的;它们的表达模式表明ABI3在干燥后的恢复阶段发挥作用.
结论:
- 布里奥菲特与血管种子共享ABA,ABI3和干燥耐受性之间的保存功能联系.
- ABI3是ABA信号通路的关键组成部分,它赋予了的干燥耐受性.
- ABI3不仅在保护作用,而且在 *Physcomitrella patens* 干燥应激后的恢复过程中发挥作用.
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