相关实验视频
Updated: May 23, 2026
07:27
Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
Published on: April 30, 2010
一个复制分叉的直接重新启动被一个正面的RNA聚合酶停滞了
Richard T Pomerantz1, Mike O'Donnell
1The Rockefeller University, Howard Hughes Medical Institute, 1230 York Avenue, New York, NY 10021, USA.
Science (New York, N.Y.)
|January 30, 2010
概括
细菌复制体在与转录复合体相碰撞时保持稳定,最终取代RNA聚合酶 (RNAP) 恢复DNA复制. Mfd蛋白质有助于这种重新启动,突出了转录合修复在复制分叉进展中的作用.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 生物化学 生物化学
背景情况:
- 复制叉可以在遇到正面转录复合体时停滞.
- 这些碰撞的精确分子事件和结果尚未完全理解.
研究的目的:
- 为了调查复制机器和RNA聚合酶 (RNAP) 在正面碰撞后的命运.
- 阐明控制复制叉稳定性和重启的机制.
主要方法:
- 在体内对大肠杆菌的研究.
- 对复制叉动态和RNA聚合酶相互作用的观察.
主要成果:
- 大肠杆菌复制体在与正面转录复合体相碰撞时停止工作.
- 复制叉保持稳定,并最终在取代RNAP后恢复延长.
- 转录-修复合因子Mfd促进RNAP的移位,并促进分叉重新启动.
结论:
- 细菌复制叉具有内在的稳定性,可以克服正面的转录阻塞.
- 通过Mfd,转录合的修复途径在重新启动停滞不前的复制分叉中起着至关重要的作用.
相关概念视频
01:02The DNA Replication Fork
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork. Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
03:01The Replisome
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
02:37Restarting Stalled Replication Forks
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
01:02The DNA Replication Fork
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork. Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
03:01The Replisome
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
02:37Restarting Stalled Replication Forks
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...