特雷斯林与TopBP1合作,触发DNA复制的启动
Akiko Kumagai1, Anna Shevchenko, Andrej Shevchenko
1Division of Biology 147-75, California Institute of Technology, Pasadena, CA 91125, USA.
Cell
|February 2, 2010
概括
特雷斯林是一种新型蛋白质,与TopBP1合作,在脊椎动物中启动DNA复制. 这种相互作用对于将Cdc45蛋白载入复制原点至关重要,这是DNA合成的关键步骤.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 遗传学 是一个遗传学.
背景情况:
- 在脊椎动物中,TopBP1对于DNA复制和检查点控制至关重要.
- 了解DNA复制的启动是细胞生物学的基础.
研究的目的:
- 识别和描述参与DNA复制启动的新型蛋白质.
- 阐明特雷斯林在DNA复制过程中的作用.
主要方法:
- 鸟蛋提取物实验研究蛋白质相互作用和DNA复制.
- 染色体结合试验. 染色体结合试验.
- 耗尽研究来评估蛋白质功能.
- 人类细胞系实验.
主要成果:
- 特雷斯林被确定为一种与Xenopus蛋提取物中的TopBP1相关的蛋白质.
- 特雷斯林的耗尽显著抑制了染色体DNA复制.
- 特雷斯林独立于TopBP1.1,与染色素结合.
- 特雷斯林对于Cdc45在染色质上加载至关重要.
- 特雷斯林和TopBP1以Cdk2依赖的方式相互作用.
- 在人体细胞中切除特雷斯林也抑制了DNA复制.
结论:
- 特斯林和TopBP1合作调节Cdk2介导的Cdc45在复制起源上的负载.
- 特雷斯林在启动脊椎动物的DNA复制中起着至关重要的作用.
相关概念视频
Translesion DNA Polymerases
Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
Restarting Stalled Replication Forks
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
The Replisome
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
The Replisome
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
Replication in Prokaryotes
DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
Replication in Prokaryotes
Overview


