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Single Cell Fate Mapping in Zebrafish
Published on: October 5, 2011
活细胞1和2光子解的远红色发射阿克里迪诺的光体
David Warther1, Frédéric Bolze, Jérémie Léonard
1Laboratoire de Conception et Application de Molécules Bioactives, UMR 7199 CNRS, Faculté de Pharmacie, Université de Strasbourg, 74 Route du Rhin 67401 Illkirch Cédex, France.
Journal of the American Chemical Society
|February 10, 2010
概括
研究人员合成了PENB-DDAO,一种新型的可光激活光体. 这种化合物能够快速有效地释放光,为先进的生物成像应用带来希望.
科学领域:
- 有机化学 有机化学
- 摄影化学的使用.
- 生物物理学的生物物理.
背景情况:
- 远红色发射光体对于生物成像至关重要.
- 可光激活的探头允许对光的时空控制.
- 开发高效的脱策略仍然是一个活跃的研究领域.
研究的目的:
- 报告PENB-DDAO的总合成和光物理特性.
- 为了评估光激活后光释放的效率和速度.
- 为了证明PENB-DDAO在活细胞中的细胞透性和光激活能力.
主要方法:
- 对PENB-DDAO分子的总合成.
- 摄影物理特征,包括脱的横截面测量.
- 使用HeLa细胞进行基于细胞的测定,以评估一光子和两光子光激活.
主要成果:
- 成功合成了PENB-DDAO,这是1,3-二-9,9-二甲基-9H-亚克里丁-2(7) - 一 (DDAO) 的衍生物.
- 实现光信号的高效 (>95%) 和快速 (<15微) 释放.
- 证明了显著的两光子解截面 (3.7GM在740nm).
- 通过一光子 (360 nm) 和两光子 (770 nm) 辐射在HeLa细胞中成功触发细胞质红色光.
结论:
- PENB-DDAO是一种高效的可光激活的远红色发射光体.
- 它的快速脱和优秀的两光子特性使其适合于先进的生物成像.
- 活细胞中的细胞透性和向光激活突出了其在体内研究的潜力.
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