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Preparation of Aplysia Sensory-motor Neuronal Cell Cultures
Published on: June 8, 2009
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阿普利西亚CPEB可以在感觉神经元中形成类似子的多元体,这有助于长期促进
Kausik Si1, Yun-Beom Choi, Erica White-Grindley
1Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MO 64110, USA. ksi@Stowers-Institute.org
Cell
|February 11, 2010
概括
蛋白 ApCPEB,像一个子,在神经元中形成自我维持的多重体. 氨酸增强了这种转化,阻断多重体形式破坏了突触记忆的持久性.
科学领域:
- 神经科学是一个神经科学.
- 分子生物学分子生物学
- 子生物学 子生物学
背景情况:
- 子是具有自我延续的结构状态的蛋白质.
- 阿普利西亚CPEB (ApCPEB) 稳定了突触功效的变化.
- 以前的研究表明,ApCPEB在酵母中表现出类似的特性.
研究的目的:
- 调查ApCPEB是否在神经系统中充当子样蛋白的功能.
- 确定血清素在ApCPEB多元化中的作用.
- 评估ApCPEB多元仪对突触可塑性持久性的影响.
主要方法:
- 在Aplysia感官神经元中,ApCPEB的外源表达.
- 对ApCPEB氨基基原多重体形成的分析.
- 对血清素对ApCPEB转换的影响的评估.
- 使用抗体阻止多重体ApCPEB并观察突触促进.
主要成果:
- 外源表达的ApCPEB在神经元中形成了自我维持的amyloidogenic多元体.
- 血清素增强了外源和内源ApCPEB的转化到多重体状态.
- 针对多重体ApCPEB的抗体抑制了突触促进的持久性.
结论:
- ApCPEB可以在神经系统中充当类似子的蛋白质.
- 这种类似子的机制可能是长期突触可塑性的基础.
- 血清激素调节ApCPEB的类活性,影响记忆的持久性.
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