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Isolation of Murine Embryonic Hemogenic Endothelial Cells
Published on: June 17, 2016
造血干细胞在发育过程中直接来自大动脉内皮
Julien Y Bertrand1, Neil C Chi, Buyung Santoso
1Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, California 92093-0380, USA.
Nature
|February 16, 2010
概括
研究人员可视化了斑马鱼的血造干细胞 (HSC) 如何从背部主动脉内皮中发展. 这项研究阐明了HSC生成,这对于再生医学和细胞疗法至关重要.
科学领域:
- 发育生物学是发展生物学.
- 干细胞生物学 干细胞生物学
- 再生医学是一种再生医学.
背景情况:
- 从诱导多能干细胞 (iPSC) 产生造血干细胞 (HSC) 是再生医学的一个关键目标,但仍然具有挑战性.
- 了解胚胎HSC发育对于推进细胞替代疗法至关重要.
- 之前的研究表明,HSCs来自于大动脉附近的血源性内皮细胞 (ECs).
研究的目的:
- 直接可视化和描述斑马鱼胚胎从背部大动脉中HSCs的体内生成.
- 为了确定从血源性内皮细胞转化为HSCs的特定细胞中间体.
- 确认这些新生成的HSCs对成年人造血系统的血统贡献.
主要方法:
- 利用斑马鱼胚胎进行体内成像,因为它们的光学透明度.
- 采用光记者转基因用于细胞跟踪和识别.
- 应用了共聚焦时隔显微镜来观察动态细胞过程.
- 使用流细胞计量用于细胞隔离和表征.
- 实施永久血统追踪来追踪细胞命运.
主要成果:
- 从背部大动脉的腹壁直接对HSCs的逐步生成进行了成像.
- 在血源性内皮转化为HSC期间识别和分离的中间细胞群.
- 证明来自血源性内皮的HSCs是成年人造血系统的创始人.
结论:
- 背部大动脉中的血源内皮是斑马鱼发育过程中HSCs的直接来源.
- 这项研究提供了HSC生成的详细地图 in vivo,为基于iPSC的疗法提供了洞察力.
- 这些发现验证了血源性内皮模型及其对理解干细胞起源的相关性.
相关概念视频
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