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在哺乳动物膜中测量了膜蛋白二聚化的能量

Lirong Chen1, Lawrence Novicky, Mikhail Merzlyakov

  • 1Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, Maryland 21218, USA.

Journal of the American Chemical Society
|February 18, 2010
PubMed
概括

研究人员开发了一种新的方法来测量哺乳动物细胞中的膜蛋白相互作用. 这种技术揭示了分子拥挤如何影响蛋白质二分化,为细胞膜动态提供了洞察力.

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Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...