相关实验视频

Updated: Jun 15, 2026

Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies

Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies

Published on: November 4, 2021

通过一个优化的型组学平台扩展双基酸酶4调节的型组

Arthur D Tinoco¹, Debarati M Tagore, Alan Saghatelian

¹Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA.

Journal of the American Chemical Society

|February 25, 2010

概括

研究人员增强了基论平台,发现了70多种脏二二酶4 (DPP4) 基质,改善了的识别并揭示了DPP4.

科学领域:

生物化学和化学生物学
分析化学分析化学
遗传学遗传学是一个

背景情况:

生物分子的定量测量在生物科学中正在迅速发展.
了解生物分子之间的调节和信号连接至关重要.
在体内识别代酶基质是理解代谢的关键.

研究的目的:

开发一种多学科的方法来在体内识别化酶基质.
优化基于液体染色学和质谱学 (LC-MS) 的基组学平台,以改善覆盖范围.
在组织中发现双基酶4 (DPP4) 的新基质.

主要方法:

采用了多学科的方法,整合了遗传学,分析化学,合成化学,生物化学和化学生物学.
采用基于液态染色体质谱 (LC-MS) 的基组学平台.
优化了关键的工作流程步骤,使用来自二二酶4淘汰赛小鼠 (DPP4(-/-) 小鼠) 和已知的基质的组织.

主要成果:

发现了70多个脏二二酶4 (DPP4) 基质,覆盖率增加了十倍.
鉴定了DPP4基质,在含的代谢中起到广泛的作用.
在肠道样本中检测到已知生物活性的数量增加,表明更广泛的平台实用性.

结论:

优化的基论平台显著提高了识别内源性基质的能力.
这些发现加强了氨基酶和DPP4活动的生物化学模型.
改进的平台具有更广泛的潜力来检测生物活性和理解生理过程.

更多相关视频

A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues

A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues

Published on: May 4, 2022

相关概念视频

Dipeptidyl Peptidase 4 Inhibitors

Dipeptidyl Peptidase 4 Inhibitors

Dipeptidyl peptidase 4 (DPP-4) is a serine protease widely distributed in the body. It's involved in the inactivation of GLP-1 and GIP hormones, which are crucial for insulin regulation. DPP-4 inhibitors, such as sitagliptin (Januvia), saxagliptin (Onglyza), linagliptin (Tradjenta), alogliptin (Nesina), and vildagliptin (Galvus), help increase the proportion of active GLP-1, enhancing insulin secretion. These inhibitors work by competitively binding to DPP-4. This binding causes a significant...

Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...