人类PAF1复合体在染色体转录延长中独立地和与SII/TFIISIS合作地起作用
Jaehoon Kim1, Mohamed Guermah, Robert G Roeder
1The Rockefeller University, New York, NY 10065, USA.
Cell
|February 25, 2010
概括
PAF1复合体 (PAF1C) 直接促进从染色质的转录延长. 它与延长因子SII/TFIIS协同工作,揭示了PAF1C在基因调节中的新作用.
科学领域:
- 分子生物学分子生物学
- 基因法规 基因法规
- 染色体动力学 染色体动力学
背景情况:
- PAF1复合体 (PAF1C) 与转录有关,但其在染色体转录中的直接作用尚不清楚.
- 之前的研究集中在PAF1C参与基因组修饰,而不是直接转录促进.
研究的目的:
- 从染色质模板中调查人类PAF1C (hPAF1C) 在转录延长中的直接作用.
- 阐明hPAF1C的功能机制及其与其他转录因子的相互作用.
主要方法:
- 生物化学定义的RNA聚合酶II转录系统.
- 重组染色体模板试验. 重组染色体模板试验.
- 对hPAF1C和延长因子SII/TFIIS相互作用的分析.
主要成果:
- hPAF1C直接促进了从染色体中取决于激活剂 (p53) 和酸转移酶 (p300) 的转录延长.
- 鉴定出了hPAF1C的一个新功能,它与基因素无处不在和甲基化不同.
- 观察到hPAF1C和延长因子SII/TFIIS之间的强烈协同作用,通过直接相互作用和与RNA聚合酶II的合作结合进行介导.
结论:
- hPAF1C在直接促进染色质的转录延长方面具有独特的功能.
- 揭示了hPAF1C和SII/TFIIS在染色体转录中的合作作用的分子机制.
- 这些发现促进了对基因表达中的转录因子合作的理解.
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