KaiC的高ATPase活性适用于Synechococcus elongatus细胞分裂的昼夜检查点
Guogang Dong1, Qiong Yang, Qiang Wang
1Center for Biological Clocks Research, Department of Biology, Texas A&M University, College Station, 77843-3258, USA.
Cell
|February 25, 2010
概括
蓝藻细菌的昼夜时钟通过调节FtsZ环形成来控制细胞分裂. 关键时钟蛋白 KaiC 的时间.
科学领域:
- 循环生物学的日间生物.
- 细胞周期调节细胞周期调节
- 微生物生理学 微生物生理学
背景情况:
- 循环时钟协调生理和行为中的日常节奏.
- 细胞周期与某些生物体的昼夜时钟有关.
- 蓝藻细菌的昼夜时钟关闭细胞分裂的机制是未知的.
研究的目的:
- 为了研究Synechococcus elongatus细胞分裂的昼夜门的机制.
- 为了确定参与调节细胞运动的时钟组件.
主要方法:
- 野生类型的Synechococcus elongatus的时间缩影显微镜.
- 对包括KaiC和KaiA蛋白质在内的关键昼夜钟组件的分析.
主要成果:
- 在野生类型的Synechococcus elongatus中证实了细胞分裂门.
- 与ATPase活性相关的KaiC蛋白的特定状态抑制了FtsZ环的形成.
- 发现了一种新型活性,可以刺激独立于KaiA的KaiC酸化.
结论:
- 昼夜时钟通过抑制FtsZ环形成来控制Synechococcus elongatus的细胞分裂.
- KaiC的ATPase活性和酸化在细胞分裂门和昼夜行为中起着不同的作用.
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