一种酵母起源的复制在S阶段晚期被激活
B M Ferguson1, B J Brewer, A E Reynolds
1Department of Genetics SK-50, University of Washington, Seattle 98195.
Cell
|May 3, 1991
概括
大型真核染色体区域因复制起源的延迟激活而迟复制,而不是缓慢的分叉运动. 这项研究揭示了原始发射控制复制模式的时间.
科学领域:
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
- 细胞生物学 细胞生物学
背景情况:
- 了解真核细胞中DNA复制时间的调节对于理解基因组稳定性和细胞周期控制至关重要.
- 已知大型染色体域在S阶段晚期复制,但基本机制在很大程度上仍未知.
研究的目的:
- 在真核染色体中研究负责端粒相邻DNA晚期复制的机制.
- 为了确定延迟的复制是由于复制叉进展缓慢或复制起源的晚期激活.
主要方法:
- 利用二维凝电泳来识别和分析复制的活性起源.
- 进行了动态分析,以测量在特定染色体区域的复制分叉运动的速度.
主要成果:
- 在Saccharomyces cerevisiae的染色体V上确定了一个67kb的端粒相邻区域,在S阶段晚期复制.
- 证实了ARS501,这个区域内的ARS元素,作为复制的活性源.
- 证明了在这个迟复制区域的复制分叉速度与早期复制区域相比较.
结论:
- 这个染色体区域的延迟复制主要是由于其复制起源 (ARS501) 的晚期激活引起的.
- 这些发现支持这样的假设,即起源激活的时间调节决定了在真核染色体中观察到的早期和晚期复制的模式.
相关概念视频
02:31Replication in Eukaryotes
Overview
02:31Replication in Eukaryotes
Overview
02:38S-Cdk Initiates DNA Replication
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
01:29Replication in Eukaryotes
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
02:38S-Cdk Initiates DNA Replication
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
01:29Replication in Eukaryotes
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...