通过对抗性转录因子控制阿拉比多普西斯的基-基底胚胎极性
1Plant Biology Laboratory, The Salk Institute for Biological Studies, University of California San Diego, La Jolla, California 92037, USA.
Nature
|March 2, 2010
概括
研究人员确定了控制植物胚胎发育的关键基因. 第三类的HOMEODOMAIN-LEUCINE ZIPPER (HD-ZIP III) 基因调节了芽的形成,而PLETHORA (PLT) 基因控制了根的发育,显示出一个对抗性的关系.
科学领域:
- 植物发育生物学植物发育生物学
- 阿拉比多普西斯的胚胎发生.
- 植物中的基因调节.
背景情况:
- 植物在胚胎发育过程中建立了一个顶点 - 基点轴,用于器官模式.
- 普莱托拉 (PLT) 基因是已知的根命运调节者,但峰/射击命运调节者是未知的.
- 托普勒斯 (TPL) 是一种参与植物发育的转录共抑制剂.
研究的目的:
- 为了确定阿拉比多普西斯胚胎发生中的顶端/射击命运的主调节者.
- 为了阐明发芽和根调节途径之间的遗传相互作用.
- 了解TOPLESS (TPL) 在轴建立中的作用.
主要方法:
- 使用Arabidopsis中的tpl-1突变体进行遗传分析.
- 对PLT和HD-ZIP III基因的基因表达分析.
- 错误表达研究,以评估基因功能在命运决定.
主要成果:
- PLT1和PLT2基因是TOPLESS (TPL) 的直接目标.
- 第三类家庭域 - 氨酸拉链 (HD-ZIP III) 转录因子被确定为顶峰命运的主调节者.
- HD-ZIP III 基因可以将胚胎根杆转化为射门杆.
- 在极子规范中,PLT和HD-ZIP III基因之间存在敌对关系.
结论:
- HD-ZIP III基因足以赋予射击命运,而PLT基因则赋予根命运.
- PLT和HD-ZIP III基因之间的相互作用对于树枝和根极的建立至关重要.
- 托普勒斯 (TPL) 在调节基底/根的命运方面,在PLT基因上游起作用.
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