通过Arabidopsis中的一个小RNA路径控制雌性性偶体的形成
Vianey Olmedo-Monfil1, Noé Durán-Figueroa, Mario Arteaga-Vázquez
1Grupo de Desarrollo Reproductivo y Apomixis, Laboratorio Nacional de Genómica para la Biodiversidad y Departamento de Ingeniería Genética de Plantas, Cinvestav Irapuato CP36500 Guanajuato, México.
Nature
|March 9, 2010
概括
阿拉比多普西斯中的ARGONAUTE 9 (AGO9) 蛋白质通过控制雌性体细胞前体来限制雌性体细胞的形成. 这个过程依赖于来自伴侣细胞的小RNA (sRNA) 沉默,这对于确定卵子中的细胞命运至关重要.
科学领域:
- 植物生殖生物学 植物生殖生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子遗传学 分子遗传学
背景情况:
- 开花植物中的雌性性体生通常从从巨母细胞 (MMC) 衍生的单个功能性巨体开始.
- 存在各种变异,一些物种和突变物种形成多个MMC或通过apomictically (没有半变异) 生产雌同体.
- 这表明卵子中的体细胞可以响应决定生育过程的信号.
研究的目的:
- 为了研究阿拉比多普西斯阿尔戈纳乌特9 (AGO9) 在控制雌性性交配体形成中的作用.
- 阐明 AGO9 调节体细胞前体特异的机制.
主要方法:
- 对阿拉比多普西斯突变的分析,特别是ago9,抑制基因沉默3 (sgs3) 和依赖RNA的RNA聚合酶6 (rdp6) 的分析.
- 在卵子组织中研究AGO9的蛋白质表达模式.
- 研究AGO9与小RNA (sRNA) 的相互作用及其起源,特别是可转移元素 (TE).
主要成果:
- 在AGO9中发生的突变导致形成能够启动游戏生殖的多个游戏细胞.
- AGO9蛋白在体相伴细胞中局部化,而不是在配子细胞系中.
- 在SGS3和RDP6中出现的缺陷显示出与ago9突变体相似的表型,突出显示了sRNA从伴侣细胞移动的重要性.
- AGO9与来自TEs的24核酸sRNA相互作用,并且对于将TEs沉默在子细胞和辅助细胞中至关重要.
结论:
- 在Arabidopsis卵子发育过程中,对确定细胞命运至关重要的是AGO9依赖的sRNA沉默.
- 在伴侣细胞中进行表观遗传重编程是必要的,以便在植物体内有效地进行sRNA依赖的沉默.
- 这种机制确保了从单个细胞谱系中正确启动雌性性体生.
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