半孔二氧化纳米颗粒大小,孔隙排序和孔隙完整性对溶血活动的影响
Yu-Shen Lin1, Christy L Haynes
1Department of Chemistry, University of Minnesota, 207 Pleasant Street SE, Minneapolis, Minnesota 55455, USA.
Journal of the American Chemical Society
|March 17, 2010
概括
多孔的二氧化纳米颗粒表现出大小依赖的毒性,但只有当它们的孔隙结构完好时. 损害毛孔完整性会增加血解,而PEG涂层可以消除它.
科学领域:
- 纳米材料的毒理学
- 纳米颗粒的生物相容性
- 细胞-纳米粒子相互作用
背景情况:
- 纳米粒子 (SiNPs) 被广泛使用,需要对毒性进行评估.
- 了解纳米粒子-细胞相互作用对于安全应用至关重要.
- 血液溶解是红细胞膜损伤的关键指标.
研究的目的:
- 为了评估多孔和无孔纳米颗粒的毒性.
- 为了研究毛孔结构和完整性对毒性的影响.
- 确定表面化学在纳米粒子诱导血解中的作用.
主要方法:
- 血液溶解试验用于测量红细胞损伤.
- 不同的纳米粒子大小,孔隙性和表面修饰.
- 纳米粒子结构和孔隙完整性的表征 (TEM,XRD,N2吸附-脱附).
主要成果:
- 无论是多孔的还是无孔的SiNPs都会引起度和大小依赖的血液溶解.
- 尺寸依赖性血液溶解仅在具有有序毛孔的中孔性SiNP中观察到.
- 多孔SiNPs显示出比无孔SiNPs更低的溶血活性.
- 损害毛孔完整性增加了血液溶解;PEG涂层消除了它.
结论:
- 孔隙结构和完整性是纳米粒子毒性的关键因素.
- 表面化学,特别是醇组,影响溶血活性.
- 用PEG进行表面修饰可以减轻纳米粒子诱导的血液溶解.
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