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Updated: Jun 15, 2026

Constructing Thioether/Vinyl Sulfide-tethered Helical Peptides Via Photo-induced Thiol-ene/yne Hydrothiolation
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在不可逆转的化物向的侧链之间自发的分子间胺基键形成

Bijan Zakeri1, Mark Howarth

  • 1Department of Biochemistry, Oxford University, South Parks Road, Oxford, OX1 3QU, UK.

Journal of the American Chemical Society
|March 19, 2010
PubMed
概括

研究人员开发了一种新的16氨基酸标签,通过氨基酸键形成不可逆转地与蛋白质结合. 这种稳定的标签为蛋白质分析,生物组装和细胞成像应用提供了新的可能性.

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Peptide Bonds02:43

Peptide Bonds

A peptide bond covalently attaches amino acids through a dehydration reaction. One amino acid's carboxyl group and another amino acid's amino group combine, releasing a water molecule. The resulting bond is the peptide bond. The products that such linkages form are peptides. As more amino acids join this growing chain, the resulting chain is a polypeptide. Each polypeptide has a free amino group at one end. This end has the N-terminal, or the amino-terminal, and the other end has a free...
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The DCC-promoted synthesis of amides begins with the protonation of DCC by carboxylic acid. The protonation makes it a better acceptor. Next, the addition of carboxylate to the protonated carbodiimide gives a reactive acylating agent.
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Amides to Carboxylic Acids: Hydrolysis

Amides can undergo either acid-catalyzed hydrolysis or base-promoted hydrolysis through a typical nucleophilic acyl substitution. Each hydrolysis requires severe conditions.
Acid-catalyzed hydrolysis:
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Amines to Amides: Acylation of Amines

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Protein Organization

Overview
Protein Organization01:24

Protein Organization

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