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指-DNA识别:Zif268-DNA复合物的晶体结构在2.1 A

N P Pavletich¹, C O Pabo

¹Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205.

Science (New York, N.Y.)

|May 10, 1991

概括

研究人员确定了与DNA结合的指的晶体结构. 这揭示了这些蛋白质如何识别DNA序列,有助于设计新的DNA结合蛋白.

科学领域:

分子生物学分子生物学
结构生物学结构生物学
遗传学是一个遗传学.

背景情况:

指图案对于调节真核生物基因表达至关重要.
了解它们的DNA结合机制是基因调节研究的关键.

研究的目的:

为了确定Zif268指DNA复合物的高分辨率晶体结构.
阐明调控指介导DNA识别的分子相互作用.

主要方法:

在2.1安格斯特罗姆分辨率的X射线晶体学.
复杂结构的完善,以达到18.2%的R系数.

主要成果:

Zif268的三个指与B-DNA的主要沟结合.
该复合体围绕着DNA双螺旋环,每个手指与一个三基对子站点相互作用.
通过一个阿尔法螺旋区域与富含瓜宁的链进行初级接触.

结论:

确定的结构为理解指DNA识别提供了详细的框架.
指图案是设计新型DNA结合蛋白质的宝贵基础.

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