发育突变的细胞外补充意味着S. coelicoloror在空中菌体形成中含有一个小的分泌蛋白
J Willey1, R Santamaria, J Guijarro
1Department of Cellular and Developmental Biology, Harvard University, Cambridge, Massachusetts.
Cell
|May 17, 1991
概括
斯特雷普托米切斯 (Streptomyces coelicolor) 的空中形形成需要蛋白质SapB. 在突变殖民地附近应用SapB蛋白恢复了空中叶的生长,确定SapB是关键的形态遗传因素.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 发育生物学 发展生物学
背景情况:
- 斑点菌 (Streptomyces coelicolor) 经历分化,形成空气菌,这些菌发展为子.
- 空中形的形成与SapB的产生有关,SapB是围绕细菌殖民地分化的蛋白质.
- 在bld突变体 (在空中形形成中被阻止) 中,SapB的产生是缺乏的,但在why突变体中 (形形成被阻止) 则不是.
研究的目的:
- 调查SapB在Streptomyces coelicolor空中形形成中的作用.
- 为了确定SapB是否是一种对空气生长至关重要的形态遗传蛋白质.
主要方法:
- 在S. coelicolor.中新发现的一种bld突变的特征.
- 评估接近SapB产生细菌对突变空气形形成的影响.
- 评估净化SapB蛋白应用对突变殖民地的影响.
主要成果:
- 在一个bld突变体中,当将其置于SapB产生细菌附近时,空气形形成被恢复.
- 将纯化的SapB蛋白直接应用于突变殖民地也恢复了空中叶的生长.
- 这些发现证实了SapB在使叶能够在空气中生长方面发挥的重要作用.
结论:
- SapB被认为是Streptomyces coelicolor空中菌体发育中的关键形态遗传蛋白.
- SapB促进了表面叶向空气生长的过渡,这是一个关键的分化步骤.
- 这项研究强调了SapB在克服S. coelicolor发育障碍方面的功能.
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