通过基因转移的假定载体载体恢复了主要的I类基因相容性分子的表达
1Division of Tumor Virology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115.
Nature
|May 23, 1991
概括
供应因子 (PSF) 对于在主要基因相容性复合体 (MHC) I 类分子上呈现抗原至关重要. 基因转移实验证明了PSF的存在.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- 细胞毒性T淋巴细胞 (CTLs) 识别由主要基因相容性复合体 (MHC) I 类分子呈现的片.
- 类载入MHC I类分子发生在内细胞网膜或相关的区间.
- 位于MHCII类区域的供应因子 (PSF) 基因与MHCI类载荷的细胞解质的运输有关.
研究的目的:
- 功能性地研究供应因子 (PSF) 在MHC I类抗原呈现途径中的作用.
- 为了确定PSF是否起到作为MHC I类分子表面表达必不可少的载体的作用.
主要方法:
- 用PSF的互补DNA (cDNA) 进行基因转移实验.
- 使用了人类淋巴细胞细胞系突变物 (721.134和721.174) 存在抗原呈现缺陷.
- 在基因转移后评估HLA-A2和-B5分子的表面表达水平.
主要成果:
- 在721.134个突变细胞系中,PSFcDNA的表达恢复了HLA-A2和-B5的正常表面水平.
- 在721.174突变体中没有观察到表面MHC I类分子的恢复,该突变体的删除包括PSF和其他基因.
- 这些发现为PSF在MHC I类抗原呈现中的作用提供了功能性证据.
结论:
- 供应因子 (PSF) 在功能上是MHC I类分子正确表面表达所需的.
- 这些结果支持了PSF作为载体的功能,可能是一种.
- 在721.174个细胞中删除区域内的额外基因也可能是完整的PSF功能在抗原呈现中所必需的.
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